产品货号：
Z38221
中文名称：
HLA-G Purified Azide Free抗体
英文名称：
Anti-HLA-G Purified Azide Free Antibody (Clone#MEM-G/9)
产品规格：
0.1mg(C100)
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-HLA-G Purified Azide Free Antibody (Clone#MEM-G/9) |
| 指标别名 | HLA 6.0;HLA G;HLA G antigen;HLAG;HLA-G;MHC class I antigen G;MHC G |
| 克隆性 | Monoclonal |
| 检验物种 | human |
| 应用范围 | FCM,IP,IHC-F,ICC,ELISA |
| 基因名称 | HLA-G |
| 克隆号 | MEM-G/9 |
| 抗体来源 | Mouse |
| 抗体类型 | IgG1 |
| 免疫原 | Recombinant human HLA-G refolded with beta2-microglobulin and peptide.The antibody MEM-G/9 reacts with an extracellular epitope on native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form.Reactivity with HLA-G3 was also reported.The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris,2003). |
| 计算分子量 | 38kDa |
| 成分 | Phosphate buffered saline (PBS),pH7.4,azide-free |
| 纯化方式 | Purified by protein-A affinity chromatography. |
| 浓度 | 1mg/mL |
| 产品形态 | 溶液 |
| 保存条件 | Store at 2～8℃.Do not freeze. |
| 背景资料 | Human leukocyte antigen G (HLA-G),belonging to MHC class I glycoproteins,plays important roles in both physiological and pathological immunotolerance.It gives an inhibitory signal to cytotoxic T cells,NK cells,monocytes,and some other immune cells.It also induces regulatory T cells and anti-inflammatory macrophages.HLA-G is important e.g.for maternal tolerance to the fetus,and for immunomodulation in particular adult tissues,such as in cornea,pancreatic islets,thymus and other.On the other hand,it is expressed in many solid and hematologic malignancies,where it contributes to evasion of the immune surveillance.HLA-G expression pattern in cancer is an important prognostic factor regarding a poor clinical outcome.Unlike most other MHC glycoproteins,HLA-G acts as an immune checkpoint molecule rather than as an antigen presenting molecule.It concerns both transmembrane and soluble HLA-G isoforms.Among other,HLA-G can promote Th2 immunological response and downregulate Th1 immunological response.For its benefits regarding allograft tolerance,including embryo implantation,soluble HLA-G (sHLA-G) can be used as a marker of developmental potential of embryos during the process of in vitro fertilization.Similarly,sHLA-G concentrations in maternal serum are decreased in preeclampsia.Transplanted patients with increased sHLA-G serum levels have improved allograft acceptance.On the other hand,increased sHLA-G can also indicate presence of malignant (sometimes also of benign) tumor cells.Another important topic is induction of HLA-G expression (sometimes associated with shedding of HLA-G from the cell surface) by some anti-cancer or anti-viral therapies,which can weaken the therapy effect.Monitoring of HLA-G in patients thus has a wide usage. |
| Uniprot ID | P17693 |
| 基因名全称 | Major histocompatibility complex,class I,G |
| 蛋白名全称 | HLA class I histocompatibility antigen,alpha chain G |
| 推荐稀释比 | Flow cytometry:1～5μg/mL;positive control:JEG-3 human choriocarcinoma cell line. Immunocytochemistry:2～5μg/mL.For fixation details see:Emadi et al.,Biotech Histochem.2022 Feb;97(2):136-142. ELISA:The antibody MEM-G/9 has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody B2M-01 or with antibody W6/32.Coating antibody 10μg/mL,detection antibody (biotin or peroxidase conjugate) 1μg/mL. Immunohistochemistry:5～10μg/mL. |
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